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KMID : 0545120070170122042
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 12 p.2042 ~ p.2045
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Inhibition of Proinflammatory Cytokine-induced Invasiveness of HT-29 Cells by Chitosan Oligosaccharide
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Nam Kyung-Soo
Kim Mee-Kyung
Shon Yun-Hee
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Abstract
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The effect of chitosan oligosaccharide (COS, 1 kDa TNF-α) in HT-29 cells. Inducible nitric oxide synthase (iNOS) expression induced by these cytokines was inhibited
by COS. COS pretreatment inhibited the invasiveness of cytokines-treated HT-29 cells through Matrigel-coated membrane
in a dose-dependent manner. COS also inhibited cytokinesinduced matrix metalloproteinase (MMP)-2 activity. This study
shows that proinflammatory cytokines induce NO production,iNOS expression, and invasiveness of human colorectal
adenocarcinoma HT-29 cells. COS pretreatment inhibited cytokines-mediated NO production, iNOS expression, and
invasiveness of HT-29 cells. These results provide sufficient information for the further development of COS as an
antitumor metastatic agent for the treatment of colon cancer.
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KEYWORD
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Chitosan oligosaccharide, invasion, matrix metalloproteinase-2, nitric oxide, proinflammatory cytokines
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